Teaching plan

Course organizer:

Arne Klungland

Location:

Demonstrations and practice Monday ¨C Wednesday: GA01 1140 Histologisalen PK-building, Domus Medica, Sognsvannsveien 9

Lectures Monday Aud A3.3067 (3rd floor, A-building Rikshospitalet), Sognsvannsveien 20

Lectures Tuesday, Wednesday and Thursday A3.3063/3064/3066 (3rd floor, A-building Rikshospitalet), Sognsvannsveien 20

Monday 15th August

0915 - 1000 Lecture 1. A historical view on molecular biology (AK)

1015 - 1100 Lecture 2. Journal presentation (VSG)

1115 - 1200 Lecture 3. Key methods in molecular biology (AK)

1230 - 1330 Demonstrations:

• Course overview
• Pippetting, sterile technique, seeding of bacteria, tranformation and calculations

• Control pipette volume (0)
• Dilution series of E. Coli (1a)
• Transformation of E. Coli (2a)

Tuesday 16th August

0915 - 1000 Lecture 4. Structure biology (BD)

1015 - 1100 Lecture 5. Bioinformatics (TR)

1115 - 1200 Lecture 6. Epigenetics (AK)

1230 ¨C 1300 Practice:

• Pick positive clones (2b)
• Initiate overnight (ON) culture for plasmidpreparation (2c)

1300 - 1600 Practice:

• Count colonies from dilution series (1b)
• Purify genomic mouse DNA from tail/ear (3a)
• Genotyping of mouse by PCR (3b)
• Immunocytochemistry (4a)

Wednesday 17th August

0815 - 1200 Practice:

• Preparation of plasmid DNA from E. Coli (2d)
• Prepare DNA for sequencing analysis (2e)

1230 - 1700 Practice:

• Restriction analysis of plasmid DNA (2f)
• PCR amplification of plasmid DNA (2g)
• Transfect mammalian cells (5a)
• Prepare gels for 2h an 2i
• Analyze PCR products by gel electrophoresis (2h)
• Analyze restriction products by gel electrophoresis (2h/i)

Thursday 18th August

1015 - 1200 Demonstrations 4-5 students in each group for demonstration of

• (i) DNA repair reactions
• (ii) DNA sequencing
• (iii) Cryo-sections
• (iv) Analyze transfected mammalian cells (4a)

1315 ¨C 1400 Lecture 7. Construction and analysis of transgenic animals (AK)

1415 ¨C 1600 Lecture Analyze results and DNA sequences

Summary