MF9210 - Laboratoriekurs i Molekylærbiologi ( Laboratory course in molecular biology) 25.1. - 29.1.2010

Course material will be handed out at the beginning of the course

Location:
Demonstrations and practice Monday – Wednesday: Histologisalen PK-building
Lectures Monday Aud A3.3067 (3rd floor, A-building Rikshospitalet).
Lectures Tuesday, Wednesday and Thursday rooms Aud A3.3067 (3rd floor, A-building Rikshospitalet).

Monday 25th January
0915 - 1000     Lecture 1. A historical view on molecular biology
1015 - 1100     Lecture 2. Journal presentation
1115 - 1200     Lecture 3. Key methods in molecular biology
1230 - 1330     Demonstrations Course overview
                          Pippetting, sterile technique, seeding of bacteria,
                          transformation and calculations
1330 - 1600     Practice Control pipette volume 
                          Dilution series of E. Coli 
                          Transformation of E. Coli 
 

Tuesday 26th January
0915 - 1000     Lecture 4. Structure biology
1015 - 1100     Lecture 5. Bioinformatics
1115 - 1200     Lecture 6. Epigenetics
1230 – 1300    Practice Pick positive clones 
                          Initiate overnight (ON) culture for plasmid
                          preparation
1300 - 1600     Practice Count colonies from dilution series  
                          Purify genomic mouse DNA from tail/ear  
                          Genotyping of mouse by PCR 
                          Immunocytochemistry 
 

Wednesday 27th January
0815 - 1200     Practice Preparation of plasmid DNA from E. Coli 
                          Prepare DNA for sequencing analysis
1230 - 1700     Practice Restriction analysis of plasmid DNA 
                          PCR amplification of plasmid DNA 
                          Transfect mammalian cells  
                          Prepare gels for 2h an 2i
                          Analyze PCR products by gel electrophoresis 
                          Analyze restriction products by gel electrophoresis

Thursday 28th January
1015 - 1200     Demonstrations 4-5 students in each group for demonstration of
                           (i) DNA repair reactions
                           (ii) DNA sequencing
                           (iii) Cryo-sections
                           (iv) Analyze transfected mammalian cells
1315 – 1400     Lecture 7. Construction and analysis of transgenic animals
1415 – 1500     Lecture 8. More methods in molecular biology; DNA repair, reporter
                           systems and RNAi

Friday 29th January
0915 - 1200     Analyze results and DNA sequences
                          Summary