Location:
Demonstrations and practice Monday – Wednesday: GA01 1140 Histologisalen PK-building, Domus Medica, Sognsvannsveien 9
Lectures Monday Aud A3.3067 (3rd floor, A-building Rikshospitalet),
Sognsvannsveien 20
Lectures Tuesday, Wednesday and Thursday A3.3063/3064/3066 (3rd floor, A-building Rikshospitalet), Sognsvannsveien 20
Program:
Monday 24. January
0915 - 1000 Lecture 1. A historical view on molecular biology
1015 - 1100 Lecture 2. Journal presentation
1115 - 1200 Lecture 3. Key methods in molecular biology
1230 - 1330 Demonstrations Course overview
Pippetting, sterile technique, seeding of bacteria, transformation and
calculations
1330 - 1600 Practice Control pipette volume
Dilution series of E. Coli
Transformation of E. Coli
Tuesday 25. January
0915 - 1000 Lecture 4. Structure biology
1015 - 1100 Lecture 5. Bioinformatics
1115 - 1200 Lecture 6. Epigenetics
1230 – 1300 Practice Pick positive clones
Initiate overnight (ON) culture for plasmid preparation
1300 - 1600 Practice Count colonies from dilution series
Purify genomic mouse DNA from tail/ear
Genotyping of mouse by PCR
Immunocytochemistry
Wednesday 26. January
0815 - 1200 Practice Preparation of plasmid DNA from E. Coli
Prepare DNA for sequencing analysis
1230 - 1700 Practice Restriction analysis of plasmid DNA
PCR amplification of plasmid DNA
Transfect mammalian cells
Prepare gels for 2h an 2i
Analyze PCR products by gel electrophoresis
Analyze restriction products by gel electrophoresis
Thursday 27. January
1015 - 1200 Demonstrations 4-5 students in each group for demonstration of
(i) DNA repair reactions
(ii) DNA sequencing
(iii) Cryo-sections
(iv) Analyze transfected mammalian cells
1315 – 1400 Lecture 7. Construction and analysis of transgenic animals
1415 – 1600 Lecture Analyze results and DNA sequences
Summary