Course organizer: Arne Klungland
Location:
Demonstrations and practice Monday – Wednesday: GA01 1140 Histologisalen PK-building, Domus MedicaLectures Monday Aud A3.3067, 3rd floor, A-building RikshospitaletLectures Tuesday, Wednesday and Thursday A3.3063/3064/3066, 3rd floor, A-building RikshospitaletPreliminary program:
Monday 30. January
0915 - 1000 Lecture 1. A historical view on molecular biology 1015 - 1100 Lecture 2. Journal presentation 1115 - 1200 Lecture 3. Key methods in molecular biology 1230 - 1330 Demonstrations Course overviewPippetting, sterile technique, seeding of bacteria, transformation and calculations1330 - 1600 Practice Control pipette volume Dilution series of E. Coli Transformation of E. ColiTuesday 31. January
0915 - 1000 Lecture 4. Structure biology1015 - 1100 Lecture 5. Bioinformatics 1115 - 1200 Lecture 6. Epigenetics 1230 – 1300 Practice Pick positive clones Initiate overnight (ON) culture for plasmid preparation 1300 - 1600 Practice Count colonies from dilution series Purify genomic mouse DNA from tail/ear Genotyping of mouse by PCR ImmunocytochemistryWednesday 1. February
0815 - 1200 Practice Preparation of plasmid DNA from E. Coli Prepare DNA for sequencing analysis 1230 - 1700 Practice Restriction analysis of plasmid DNA PCR amplification of plasmid DNA Transfect mammalian cells Prepare gels for 2h an 2iAnalyze PCR products by gel electrophoresis Analyze restriction products by gel electrophoresisThursday 2. February
1015 - 1200 Demonstrations 4-5 students in each group for demonstration of(i) DNA repair reactions(ii) DNA sequencing(iii) Cryo-sections(iv) Analyze transfected mammalian cells 1315 – 1400 Lecture 7. Construction and analysis of transgenic animals 1415 – 1600 Lecture Analyze results and DNA sequencesSummary