Literature to read:
Chapter. 3 (Fundamental mass spectrometry) in the book "Protein sequencing and identification using tandem mass spectrometry" by Michael Kinter og Nicholas E. Sherman, Wiley, 2000.
Abian, J.; Oosterkamp, A. J.; Gelpi, E. Comparison of conventional, narrow-bore and capillary liquid chromatography/mass spectrometry for electrospray ionization mass spectrometry: practical considerations. Journal of Mass Spectrometry (1999), 34, 244-254
Zimmer, D.. Introduction to quantitative liquid chromatography-tandem mass spectrometry (LC-MS-MS). Chromatographia (2003), 57(Suppl.), S/325-S/332.
Capiello, A., Famiglini, G., Palma P., Trufelli, H. Matrix effects in liquid chromatography-mass spectrometry. Journal of Liquid Chromatography & Related Technologies (2010), 33, 1067-1081.
Additionally you may have a look at:Smyth, W. Franklin; Brooks, Peter. A critical evaluation of high performance liquid chromatography-electrospray ionisation-mass spectrometry and capillary electrophoresis-electrospray-mass spectrometry for the detection and determination of small molecules of significance in clinical and forensic science. Electrophoresis (2004), 25(10-11), 1413-1446.
Laboratory exercises and evaluation:
The supervisor will show and explain the features of the micro-LC system coupled to an electrospray MS instrument (TOF instrument). Introduction to MassLynx software,and washing and maintenance of ESI, will be given. Thereafter the student must make a gradient method, which separates the compounds and give good response in the MS, for determination of test compounds which are provided. To pass the module the student must show TIC and RIC chromatograms of the separation and mass spectra of the compounds provided. At the end of the module, the student will be examined with regard to choosing mobile phases and MS parameters, and in the knowledge of the MS instrument and ability to transfer data to Word documents. Additionally, a 1-2 pages report must be written and approved to pass the module.
Schedule:
The module starts with a 2 hrs lecture Wednesday Oct. 31 at 10.15 (?108). Thereafter, all activites will take place in the laboratory (?122).
Requirements:
You must have taken either KJM4420, KJM3420, or KJM3400 (or an equivalent course) to participate in this module.
To be able to take this module, you must also have taken module no. 7.