Location:
Rikshospitalet, Sognsvannsveien 20
Lectures Monday: Aud A3.3067 (3rd floor, A-building Rikshospitalet).
Demonstrations and practice Monday – Wednesday: RH B1.1012A Kurssal
and Kurssal B1.1016A RH
Programme:
Monday 23. August
0915 - 1000 Welcome Presentation of course participants
Lecture 1. A historical view on molecular biology
1015 - 1100 Lecture 2. Journal presentation
1115 - 1200 Lecture 3. Key methods in molecular biology
1230 - 1330 Demonstrations Course overview
Pippetting, sterile technique, seeding of bacteria, transformation and calculations
1330 - 1600 Practice Control pipette volume
Dilution series of E. Coli
Transformation of E. Coli
Tuesday 24. August
0915 - 1000 Lecture 4. Structure biology
1015 - 1100 Lecture 5. Bioinformatics
1115 - 1200 Lecture 6. Epigenetics
1230 – 1300 Practice Pick positive clones
Initiate overnight (ON) culture for plasmid preparation
1300 - 1600 Practice Count colonies from dilution series
Purify genomic mouse DNA from tail/ear
Genotyping of mouse by PCR
Immunocytochemistry
Wednesday 25. August
0815 - 1200 Practice Preparation of plasmid DNA from E. Coli
Prepare DNA for sequencing analysis
1230 - 1700 Practice Restriction analysis of plasmid DNA
PCR amplification of plasmid DNA
Transfect mammalian cells
Prepare gels for 2h an 2i
Analyze PCR products by gel electrophoresis
Analyze restriction products by gel electrophoresis
Thursday 26. August
1015 - 1200 Demonstrations 4-5 students in each group for demonstration of
(i) DNA repair reactions
(ii) DNA sequencing
(iii) Cryo-sections
(iv) Analyze transfected mammalian cells
1315 – 1400 Lecture 7. Construction and analysis of transgenic animals
1415 – 1500 Lecture 8. More methods in molecular biology; DNA repair, reporter systems and RNAi
Friday 27. August
0915 - 1200 Analyze results and DNA sequences
Summary