Course organizer:
Arne Klungland, Professor, Oslo Universitetssykehus, Seksjon for Molekyl?rmedisinLecturers:
Arne Klungland (AK), Professor, Oslo Universitetssykehus, Seksjon for Molekyl?rmedisin Vibeke Sundvold Gjerstad (AS), Forsker, UiO, Institutt for Medisinske Basalfag, Seksjon for Anatomi Bj?rn Dalhus (BD), Forsker, CMBN, Rikshospitalet, Seksjon for Klinisk BiokjemiLocation:
The course takes place at Rikshospitalet Demonstrations and practice Monday – Wednesday: Rom: RH B1.1012A Rikshospitalet Lectures Monday seminar rooms A3.3063/3064/3066 (3rd floor, A-building Rikshospitalet) Lectures Tuesday, Wednesday and Thursday A3.3067 (3rd floor, A-building Rikshospitalet).Monday 28. January
0915 - 1000 Lecture 1. A historical view on molecular biology (AK) 1015 - 1100 Lecture 2. Journal presentation (AS) 1115 - 1200 Lecture 3. Key methods in molecular biology (AK) 1230 - 1330 Demonstrations Course overview Pippetting, sterile technique, seeding of bacteria, transformation and calculations 1330 - 1600 Practice Control pipette volume Dilution series of E. Coli Transformation of E. ColiTuesday 29. January
0915 - 1000 Lecture 4. Structure biololy (BD) 1015 - 1100 Lecture Demonstration - Structure analysis mm 1115 - 1200 Lecture 5. Epigenetics (AK) 1230 – 1330 Practice Pick positive clones Initiate overnight (ON) culture for plasmid preparation 1330 - 1600 Practice Count colonies from dilution series Purify genomic mouse DNA from tail/ear Genotyping of mouse by PCR ImmunocytochemistryWednesday 30. January
0815 - 1200 Practice Preparation of plasmid DNA from E. Coli Prepare DNA for sequencing analysis 1230 - 1700 Practice Restriction analysis of plasmid DNA PCR amplification of plasmid DNA Transfect mammalian cells Prepare gels for 2h an 2i Analyze PCR products by gel electrophoresis Analyze restriction products by gel electrophoresisThursday 31. January
0915 – 1200 Demonstrations 4-5 students in each group (i) Immunocytochemistry (ii) Analyze transfected mammalian cells Analyze results and DNA sequences Summary